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Supplementary material The Supplementary Material for this article can be found online at: References 1 VangapanduHVHavranekOAyresMLKaipparettuBABalakrishnanKWierdaWGet al

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Number of Vials:4 Vials (Differentiated Results)

At the time of analysis, cell extracts were resuspended in 80% methanol with or without U- 13 C yeast extract (Cambridge Isotope, ISO1), with or without NEM and/or isotopically labelled CYS metabolite standard mix at a concentration of 28–40 μl solvent per 1 μl cell volume, vortexed at 4 °C for 5 min and centrifuged at 17,000 g for 5 min at 4 °C

essential amino acid that helps to maintain overall good health by facilitating the transfer of fatty acid groups into the mitochondrial membrane for cellular energy production